RSV-evolution-neut

RSV Evolution Neutralization Project

Plots for for Simonich et al (2025), a study on RSV evolution from the Bloom lab.

Methods

Study done by Cassie Simonich and Teagan McMahon starting in 2024. This study validates a pseudovirus based neutralization assay developed by the authors and also looks at how well mAbs and human serum retain their neutralization as RSV evolves over time. See repo for full analysis.

Relevant Figures

• for titer plots see
  • G CT deletion 1B
  • F CT deletion 1C
  • Pseudovirus titer TU/mL 2A
  • Pseudovirus titer RLU/uL 2B
  • Freezing conditions Supplemental 1
  • 293T vs 293T-TIM1 Supplemental 2B
• for compaison of neutralization on 293T vs 293T-TIM1 cells see:
  • Nirsevimab 2C
  • Pooled sera 2C
  • Clinical serum 2C
• for validation against full length A2 assay and refernce/standard sera see:
  • Compaison to A2 assay 3A
  • Compaison to A2 assay curves Supplemental 4A
  • BEI sera curves 3B
  • NIBSC & BEI sera additional curves Supplemental 5A
  • NIBSC & BEI sera additional curves Supplemental 5A
  • BEI comparison to published values 3C
  • Dilution of virus impacts neutralization RSV B N201S Supplemental 3
  • Dilution of virus impacts neutralization RSV B 1982 Supplemental 3
• for depleted sera neuts/ELISAs see:
  • ELISA with RSV G, F, Pre-F 4B
  • Neutralization assay with depleted sera 4C
• for RSV F trees see:
  • RSV-A-F tree used in 5A
  • RSV-B-F tree used in 5B and 7A
• for historical sera NT50s and curves see:
  • RSV A and B historical sera NT50s 6B
  • RSV A and B historical sera curves Supplemental 6
  • Influenza H3 historical sera NT50s
  • Influenza H3 historical sera curves Supplemental 7 replicate 1
  • Influenza H3 historical sera curves Supplemental 7 replicate 2
  • RSV B N201S sera NT50s 7C
  • RSV B N201S sera curves Supplemental 9
• for mAb curves see:
  • mAbs curves for non lab adapted strains 7B
  • mAbs curves for lab adapted strains replicate 1 Supplemental 8A
  • mAbs curves for lab adapted strains replicate 2 Supplemental 8B
  • mAbs curves for non lab adapted strains replicate 2 Supplemental 8C