Omicron RBD DMS

Overview

We performed deep mutational scans to measure the impact on ACE2 binding of all single amino-acid mutations in the Omicron BA.1 and BA.2 variant RBDs, and compare mutational effects to those in the ancestral Wuhan-Hu-1 RBD background. Find details in the our preprint here. We combine this with other variant RBD backgrounds described in this prior publication.

Here, we link to two interactive visualizations that enable exploration of the data from these deep mutational scans across SARS_CoV-2 variants.

Instructions

We have made two tools to help visualize the data from our deep mutational scans:

  1. A set of interactive heatmaps that you can use to explore the change in ACE2-binding affinity (\(\Delta\)log10 \(K_D\)) or the change in RBD expression (log10(MFI)) caused by mutations in each RBD variant. To use this tool, click here.

  2. An interactive widget that you can use to visualize epistatic shifts in mutational effects on ACE2-binding affinity (-log10 \(K_D\)) between variant RBDs. To use this tool, click here.

Data

If you are interested in the raw data from our study, you can find the ACE2-binding affinity (-log10 \(K_D\)) and RBD expression (log10(MFI)) measurements from each experiment here. You can find the data used to plot the epistatic shifts between variant backgrounds here. Data for pre-Omicron variants (Alpha, Beta, Delta, Eta, and associated Wuhan-Hu-1 measurements) are described in this paper and found on this repository.