Near real-time data on the human neutralizing antibody landscape to influenza virus to inform vaccine-strain selection for the Northern Hemisphere in February 2026

Briefly, this study used sequencing-based neutralization assays to measure titers to influenza viruses with HAs from seasonal H3N2 and H1N1 viruses representative of those circulating in the fall-winter of 2025-2026 against a set of human sera collected in 2025.

The numerical data and computer code are at https://github.com/jbloomlab/flu-seqneut-2025to2026

Documentation of results rendered as of Sat Feb 14 17:25:06 2026

Contents

Titers for all sera

Interactive chart of titers

Per-serum neutralization titers

human

Per-plate counts and curve fits

human

Summary of data dropped during quality control

Notebook summarizing QC drops

Interactive charts summarizing human titers (vertical)

Interactive charts summarizing human titers (horizontal)

Fold-change charts

titer change after vaccination for PENN cohort (vertical)

titer change after vaccination for PENN cohort (horizontal)