Pleiotropic mutational effects on function and stability constrain the antigenic evolution of influenza hemagglutinin

In a new study led by grad student Tim Yu and posted on bioRxiv today, we used pseudovirus deep mutational scanning to measure how all mutations to a recent H3N2 HA affect cell entry, stability, and antibody escape. The results help shed light on how the pleiotropic effects of mutations shape influenza evolution.

For accessible summaries of the study, see:

• This Bluesky thread, which can be read more easily in threaded form here.
• This X thread

All the data are available for interactive visualization and download at https://dms-vep.org/Flu_H3_Massachusetts2022_DMS/