Documentation for dms_tools2¶
dms_tools2 is a software package for analyzing deep mutational scanning data. It is tailored to analyze libraries created using comprehensive codon mutagenesis of protein-coding genes, and perform analyses that are common to the Bloom lab, such as:
Process deep-sequencing data from Barcoded-subamplicon sequencing.
Estimate the Amino-acid preferences of a protein.
Estimate Differential selection when imposing a pressure such as antibody selection.
Estimate the Fraction surviving for each mutation when imposing a strong pressure such as antibody selection.
Processing PacBio CCS sequencing of defined amplicons using the
dms_tools2.pacbiomodule.
The dms_tools2 source code is freely available under a GPLv3 license. dms_tools2 is a re-write of the earlier dms_tools package.
If you use dms_tools2 for your work, please cite the references in Citations.
Installation of dms_tools2 installs a collection of Executable programs as well as a Python API.
The easiest way to learn to use dms_tools2 is to look at the Examples.
