Mutational escape from the polyclonal antibody response to SARS-CoV-2 infection is largely shaped by a single class of antibodies

Analysis of mutational antigenic profiling of SARS-CoV-2 RBD against monoclonal antibodies and convalescent patient sera from a NYC cohort.

View the Project on GitHub jbloomlab/SARS-CoV-2-RBD_MAP_Rockefeller

Interactive structural visualizations of mutational escape from three structural classes of anti-SARS-CoV-2 RBD antibodies and polyclonal plasma

Below are links to interactive visualizations of the effects of single mutations to the SARS-CoV-2 spike receptor-binding domain (RBD) on binding by monoclonal antibodies of three structural classes and polyclonal convalescent human sera.

These visualizations are built using dms-view. These experiments are described in this preprint, and raw data are available here. Monoclonal antibodies and polyclonal plasma were isolated from SARS-CoV-2 convalescent individuals, as described in Robbiani, et al. (2020).

Sites where mutations reduce binding are shown for each monoclonal antibody or polyclonal plasma, and are mapped to the ACE2-bound RBD structure (PDB 6M0J).

dms-view renderings for each monoclonal antibody’s escape mapped to the ACE2-bound RBD structure:

dms-view renderings for each antibody’s escape mapped to its respective antibody-bound spike trimer or isolated RBD structure (structures published in Barnes, et al. (2020a); Barnes, et al. (2020b); Shi, et al. (2020)). Some antibodies have more than one published structure:

dms-view renderings for each polyclonal plasma’s escape mapped to the ACE2-bound RBD structure:

dms-view links for a larger cohort of SARS-CoV-2 convalescent individuals from Greaney, et al. (2021) can be found here. These individuals were recruited for the HAARVI study in the Seattle, WA area.

*Results for LY-CoV016 were first reported in Starr et al. (2021).