Prospective mapping of viral mutations that escape antibodies used to treat COVID-19

Mutational antigenic profiling of SARS-CoV-2 RBD against REGN-COV2 and LY-CoV016

View the Project on GitHub jbloomlab/SARS-CoV-2-RBD_MAP_clinical_Abs

Interactive structural visualizations of escape mutation maps

Below are links to interactive visualizations of the effects of single mutations to the SARS-CoV-2 spike receptor-binding domain (RBD) on recognition by therapeutic antibodies used to treat COVID-19. These visualizations are built using dms-view. These experiments are described in this paper, and raw data is available here.

Sites of escape are shown for each antibody, and are mapped to the ACE2-bound RBD structure (PDB 6M0J or antibody-bound structures (Regeneron antibodies: PDB 6XDG, LY-CoV016: PDB 7C01). For single-antibody bound RBD structures, irrelevant chains were removed from the original pdb file (e.g., REGN10987 chains removed for the REGN10933-specific view)).

dms-view renderings for each antibody’s escape mapped to the ACE2-bound RBD structure:

dms-view renderings for each antibody’s escape mapped to its respective antibody-bound RBD structure: