Below are links to interactive visualizations of the effects of single mutations to the Delta SARS-CoV-2 spike receptor-binding domain (RBD) on recognition by polyclonal plasmas from individuals who were infected with the Delta variant.
These visualizations are built using dms-view. These experiments are described in this pre-print, and raw data are available here.
Sites of escape are shown for each antibody, and are mapped to the ACE2-bound Wuhan-Hu-1 RBD structure (PDB 6M0J).
These interactive dms-view renderings show mutations that reduce binding of polyclonal plasmas to the Delta RBD.
Here are the same data, but mapped to the ACE2-bound Delta RBD (PDB 7V8B).
Below are previously-published interactive dms-view renderings for early 2020 plasmas. These plasmas were mapped against DMS libraries of the homologous RBD of exposure – i.e., the Wuhan-Hu-1 RBD.
dms-view links for a cohort of early 2020 SARS-CoV-2 convalescent individuals from Greaney, et al. (2021) can be found here. Participants were recruited for the HAARVI study in the Seattle, WA area.dms-view links for a small number of early 2020 SARS-CoV-2 convalescent individuals from Greaney, et al. (2021) can be found here. Participants were recruited in the New York area and these samples were first described in Robbiani, et al. (2020).Below are previously-published interactive dms-view renderings for primary Beta infection-elicited plasmas. These plasmas were mapped against DMS libraries of the homologous RBD of exposure – i.e., the Beta RBD.
dms-view links for a cohort of SARS-CoV-2 Beta-convalescent individuals from Greaney, et al. (2022) can be found here. Participants were recruited in South Africa in late 2020-early 2021. These plasmas were first described in Cele, et al. (2021).